Hotspot

trnS-GCU-trnG-UCC

LSC · 8433-9408 · 976 bp

Back to Paphiopedilum

Pi 0.036
MarkerSeek score 82.00
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
976
variable_sites
275
pi
0.036
unique_haplotype_count
62
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA AATGGAAAGACCGCTTAACT 50.0 40.0 58.0 55.0 1083-1597 bp 1.0 67.6
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC AATGGAAAGACCGCTTAACT 50.0 40.0 58.0 55.0 1082-1596 bp 1.0 67.6
trnS-GCU-trnG-UCC_p3 CTTTAGTCCACTCAGCCACC AATGGAAAGACCGCTTAACT 55.0 40.0 57.9 55.0 1073-1587 bp 1.0 66.9
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA TGGAAAGACCGCTTAACTATTGA 50.0 39.1 58.0 57.8 1081-1595 bp 0.985075 66.1
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC TGGAAAGACCGCTTAACTATTGA 50.0 39.1 58.0 57.8 1080-1594 bp 0.985075 66.1