Hotspot

trnK-UUU-rps16

LSC · 4534-5405 · 872 bp

Back to Paphiopedilum

Pi 0.0363
MarkerSeek score 86.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
872
variable_sites
246
pi
0.0363
unique_haplotype_count
63
primer_available
yes
alignment_reliability
0.97
markerseek_score
86.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 863-1273 bp 0.985075 76.7
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 862-1272 bp 0.985075 76.7
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TGCTCAACCCACAGGAACT 50.0 52.6 58.2 58.8 862-1272 bp 1.0 76.5
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TGCTCAACCCACAGGAACT 50.0 52.6 58.2 58.8 861-1271 bp 1.0 76.5
trnK-UUU-rps16_p5 TACCGTTGAGTTAGCAACCC GTGCTCAACCCACAGGAAC 50.0 57.9 57.5 58.7 849-1259 bp 0.985075 74.4