Hotspot

ycf1

SSC · 123459-128810 · 5352 bp

Back to Papaver

Pi 0.0306

MarkerSeek score 66.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5352
variable_sites: 467
pi: 0.0306
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CAATCCTCATTCCACTCCGG	AACAAGAATGCCTGCCTGAA	55.0	45.0	58.0	58.0	979-985 bp	0.866667	82.5
ycf1_p2	CCAATCCTCATTCCACTCCG	AACAAGAATGCCTGCCTGAA	55.0	45.0	58.0	58.0	980-986 bp	0.8	78.7
ycf1_p3	TTTTCCTTTGCCTCTTCCCC	AACAAGAATGCCTGCCTGAA	50.0	45.0	58.0	58.0	775 bp	0.666667	67.3
ycf1_p4	CTGCTAGCCGGTCATTTTCT	AACAAGAATGCCTGCCTGAA	50.0	45.0	58.0	58.0	822 bp	0.666667	67.1
ycf1_p5	TTTCTCCGTCTTGTTGCTCC	AACAAGAATGCCTGCCTGAA	50.0	45.0	58.1	58.0	396 bp	0.666667	66.5