Hotspot

rpl32-trnL-UAG

SSC · 113352-113933 · 582 bp

Back to Papaver

Pi 0.0568

MarkerSeek score 77.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 582
variable_sites: 84
pi: 0.0568
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GAATACGATGAGCCGTAGCC	TTTAGAAGATGCCGCCACTC	55.0	50.0	58.3	58.0	1130-1176 bp	0.933333	86.6
rpl32-trnL-UAG_p2	ACTTTACGATATCCGGCCCT	TTTAGAAGATGCCGCCACTC	50.0	50.0	58.3	58.0	1412-1424 bp	0.666667	66.1
rpl32-trnL-UAG_p3	TACGATATCCGGCCCTTTGA	TTTAGAAGATGCCGCCACTC	50.0	50.0	58.3	58.0	1408-1420 bp	0.666667	66.1
rpl32-trnL-UAG_p4	ACTCTATGGGACTCACACGT	TTTAGAAGATGCCGCCACTC	50.0	50.0	57.8	58.0	998-1002 bp	0.666667	65.7
rpl32-trnL-UAG_p5	ACTCTATGGGACTCACACGT	CTACCGATTCCACCATAGCG	50.0	55.0	57.8	57.9	931-935 bp	0.666667	65.4