Hotspot

ndhF-rpl32

SSC · 112136-113177 · 1042 bp

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Pi 0.0429

MarkerSeek score 75.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1042
variable_sites: 127
pi: 0.0429
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAACGAAAGGT	GTACGTTTCTTTGGAACGGC	50.0	50.0	57.7	57.7	1116-1165 bp	1.0	86.5
ndhF-rpl32_p2	GCCCCACCCCTATTGCTATA	GTACGTTTCTTTGGAACGGC	55.0	50.0	58.6	57.7	1140-1189 bp	1.0	83.4
ndhF-rpl32_p3	CTGGAAGTGGAACGAAAGGT	TACGTTTCTTTGGAACGGCC	50.0	50.0	57.7	58.8	1115-1164 bp	1.0	82.7
ndhF-rpl32_p4	GAACAAGGAGAAGCCCCAC	GTACGTTTCTTTGGAACGGC	57.9	50.0	58.1	57.7	1152-1201 bp	1.0	80.0
ndhF-rpl32_p5	GCCCCACCCCTATTGCTATA	TACGTTTCTTTGGAACGGCC	55.0	50.0	58.6	58.8	1139-1188 bp	1.0	79.7