Hotspot

trnN-GUU-ndhF

IRb · 108064-109915 · 1852 bp

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Pi 0.0203

MarkerSeek score 60.10

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1852
variable_sites: 107
pi: 0.0203
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	ATGTAGGGGGCGGAAGAATA	50.0	50.0	58.2	57.9	1411-2000 bp	1.0	86.7
trnN-GUU-ndhF_p2	AGGATTCGAACCTACGACCA	TGTAGGGGGCGGAAGAATAT	50.0	50.0	58.2	57.9	1410-1999 bp	1.0	86.7
trnN-GUU-ndhF_p3	AGGATTCGAACCTACGACCA	AAAAATGTAGGGGGCGGAAG	50.0	50.0	58.2	58.2	1415-2004 bp	1.0	86.4
trnN-GUU-ndhF_p4	AGGATTCGAACCTACGACCA	AGGGGGCGGAAGAATATCTT	50.0	50.0	58.2	58.2	1407-1996 bp	1.0	86.2
trnN-GUU-ndhF_p5	AGGATTCGAACCTACGACCA	ATCAAAAATGTAGGGGGCGG	50.0	50.0	58.2	58.2	1418-2007 bp	0.933333	82.0