Hotspot

petA-psbJ

LSC · 62476-63528 · 1053 bp

Back to Papaver

Pi 0.0432

MarkerSeek score 74.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1053
variable_sites: 125
pi: 0.0432
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GTGGATTTGGTCAGGGAGAC	AACTGGCCGTTTTGATCCTT	55.0	45.0	57.9	58.0	1787-1854 bp	1.0	86.4
petA-psbJ_p2	GTGGATTTGGTCAGGGAGAC	ACTGGCCGTTTTGATCCTTT	55.0	45.0	57.9	58.0	1786-1853 bp	1.0	86.4
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	ACTGGCCGTTTTGATCCTTT	50.0	45.0	57.8	58.0	1730-1797 bp	1.0	86.2
petA-psbJ_p4	TTTGAACGTAGGGGCTGTTC	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.1	58.0	2284-2351 bp	1.0	85.7
petA-psbJ_p5	TTTGAACGTAGGGGCTGTTC	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.1	58.0	2283-2350 bp	1.0	85.7