Hotspot

trnF-GAA-ndhJ

LSC · 47637-48386 · 750 bp

Back to Papaver

Pi 0.0322
MarkerSeek score 69.50
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
750
variable_sites
74
pi
0.0322
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TGAAATACAAGATGCTCATTGA 50.0 31.8 58.1 53.8 775-887 bp 1.0 51.0
trnF-GAA-ndhJ_p2 AAGGGTTGGGATAGCTCAGT TGAAATACAAGATGCTCATTGA 50.0 31.8 58.0 53.8 818-930 bp 1.0 50.7
trnF-GAA-ndhJ_p3 AGGGTTGGGATAGCTCAGTT TGAAATACAAGATGCTCATTGA 50.0 31.8 58.0 53.8 817-929 bp 1.0 50.7
trnF-GAA-ndhJ_p4 GGGTTGGGATAGCTCAGTTG TGAAATACAAGATGCTCATTGA 55.0 31.8 58.0 53.8 816-928 bp 1.0 50.7
trnF-GAA-ndhJ_p5 GGTTGGGATAGCTCAGTTGG TGAAATACAAGATGCTCATTGA 55.0 31.8 58.0 53.8 815-927 bp 1.0 50.7