Hotspot

trnE-UUC-trnT-GGU

LSC · 30987-31437 · 451 bp

Back to Papaver

Pi 0.0453

MarkerSeek score 74.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 451
variable_sites: 58
pi: 0.0453
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	538-557 bp	1.0	89.6
trnE-UUC-trnT-GGU_p2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	536-555 bp	1.0	89.6
trnE-UUC-trnT-GGU_p3	GTAGTACCCGTACCCTACCC	GAACCGATGACTTACGCCTT	60.0	50.0	58.0	58.0	594-601 bp	0.666667	67.4
trnE-UUC-trnT-GGU_p4	GTAGTACCCGTACCCTACCC	TACCGCTGAGTTAAAAGGGC	60.0	50.0	58.0	57.9	559-566 bp	0.666667	66.7
trnE-UUC-trnT-GGU_p5	GTAGTACCCGTACCCTACCC	CCGATGACTTACGCCTTACC	60.0	55.0	58.0	58.2	591-598 bp	0.666667	66.3