Hotspot

rps16-trnQ-UUG

LSC · 5909-6989 · 1081 bp

Back to Papaver

Pi 0.0366
MarkerSeek score 78.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1081
variable_sites
111
pi
0.0366
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CCACAACCGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1175-1207 bp 1.0 90.0
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1154-1186 bp 1.0 89.9
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1165-1197 bp 1.0 89.3
rps16-trnQ-UUG_p4 CCACAACCGATCATGTCCTT CTCGGAGGTTCGAATCCTTC 50.0 55.0 57.9 57.8 1179-1211 bp 1.0 88.9
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 1158-1190 bp 1.0 88.8