Hotspot

trnH-GUG-psbA

LSC · 118-360 · 243 bp

Back to Papaver

Pi 0.0503

MarkerSeek score 67.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 243
variable_sites: 35
pi: 0.0503
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TCACAATCCACTGCCTTCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.1	57.9	323-328 bp	0.666667	69.1
trnH-GUG-psbA_p2	TCACAATCCACTGCCTTCAC	GTTGACAGTCAAGGTCGTGT	50.0	50.0	58.1	58.1	419-424 bp	0.666667	68.3
trnH-GUG-psbA_p3	TCACAATCCACTGCCTTCAC	TTGTAGCCGCTCATGGTTAC	50.0	50.0	58.1	58.0	595-600 bp	0.666667	67.8
trnH-GUG-psbA_p4	TCACAATCCACTGCCTTCAC	ATATTCCGCTCCTGTTGCAG	50.0	50.0	58.1	58.0	900-905 bp	0.666667	67.0
trnH-GUG-psbA_p5	TCACAATCCACTGCCTTCAC	CCCAATTGGTCAAGGAAGCT	50.0	50.0	58.1	58.1	855-860 bp	0.666667	66.8