Hotspot

rpl16-rps3

LSC · 80479-80625 · 147 bp

Back to Panicum

Pi 0.0503

MarkerSeek score 72.70

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 147
variable_sites: 19
pi: 0.0503
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAATCCGATGCCGTCATAGG	TGCATCAAAAAGGGCAGACT	50.0	45.0	57.8	58.0	856-900 bp	0.857143	80.9
rpl16-rps3_p2	CGCGGGCGAATATTTACTCT	TGCATCAAAAAGGGCAGACT	50.0	45.0	58.2	58.0	976-1020 bp	0.857143	80.2
rpl16-rps3_p3	TCGCGGGCGAATATTTACTC	TGCATCAAAAAGGGCAGACT	50.0	45.0	58.2	58.0	977-1021 bp	0.857143	80.1
rpl16-rps3_p4	AATCCTGCAATGGAGCTTCC	TGCATCAAAAAGGGCAGACT	50.0	45.0	58.2	58.0	795-839 bp	0.714286	74.2
rpl16-rps3_p5	CATCCCACCCAATGAAGTGT	TGCATCAAAAAGGGCAGACT	50.0	45.0	57.8	58.0	893-937 bp	0.714286	72.6