Hotspot

rpl14-rpl16

LSC · 78879-78987 · 109 bp

Back to Panicum

Pi 0.0428

MarkerSeek score 79.90

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 109
variable_sites: 14
pi: 0.0428
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	GATTACCAGCGGCTCCTATG	TAGCTGCCAGTAAAATGCCC	55.0	50.0	57.9	58.2	238-253 bp	1.0	85.9
rpl14-rpl16_p2	ATTACCAGCGGCTCCTATGA	TAGCTGCCAGTAAAATGCCC	50.0	50.0	58.3	58.2	237-252 bp	1.0	84.3
rpl14-rpl16_p3	TGATTACCAGCGGCTCCTAT	TAGCTGCCAGTAAAATGCCC	50.0	50.0	58.3	58.2	239-254 bp	1.0	84.3
rpl14-rpl16_p4	GATTACCAGCGGCTCCTATG	GCCAGTAAAATGCCCATACG	55.0	50.0	57.9	57.2	233-248 bp	1.0	81.7
rpl14-rpl16_p5	GATTACCAGCGGCTCCTATG	CCGAAACTGTAGCTAGAGCA	55.0	50.0	57.9	57.1	268-283 bp	1.0	79.8