Hotspot

trnV-UAC-trnM-CAU

LSC · 53122-53324 · 203 bp

Back to Panicum

Pi 0.0377

MarkerSeek score 75.00

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 203
variable_sites: 20
pi: 0.0377
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	CGAGTTGCTCTACCGAACTG	CCCGCCGTATGAAAGCAATA	55.0	50.0	58.4	58.4	262-304 bp	1.0	83.3
trnV-UAC-trnM-CAU_p2	CGAGTTGCTCTACCGAACTG	CCGCCGTATGAAAGCAATAC	55.0	50.0	58.4	57.3	261-303 bp	1.0	81.3
trnV-UAC-trnM-CAU_p3	CCGAACTGAGCTATAGCCCT	CCCGCCGTATGAAAGCAATA	55.0	50.0	58.7	58.4	250-292 bp	1.0	81.3
trnV-UAC-trnM-CAU_p4	CGAGTTGCTCTACCGAACTG	TTTGAACCAATGACTCCCGC	55.0	50.0	58.4	58.8	277-319 bp	1.0	80.3
trnV-UAC-trnM-CAU_p5	CCGAACTGAGCTATAGCCCT	CCGCCGTATGAAAGCAATAC	55.0	50.0	58.7	57.3	249-291 bp	1.0	79.3