Hotspot

trnC-GCA-rpoB

LSC · 19835-20893 · 1059 bp

Back to Panicum

Pi 0.0221

MarkerSeek score 67.00

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1059
variable_sites: 63
pi: 0.0221
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	TGCCCCGCTTTCTATCTTTC	CCCCACCTACACAAGCAAAT	50.0	50.0	58.0	58.1	2328-2477 bp	1.0	86.6
trnC-GCA-rpoB_p2	CTGCCCCGCTTTCTATCTTT	CCCCACCTACACAAGCAAAT	50.0	50.0	58.0	58.1	2329-2478 bp	1.0	86.6
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	CCCCACCTACACAAGCAAAT	50.0	50.0	58.1	58.1	1761-1916 bp	1.0	86.3
trnC-GCA-rpoB_p4	TGCCGACAAAACAAACCCTA	CCCCACCTACACAAGCAAAT	45.0	50.0	57.9	58.1	1959-2098 bp	0.857143	80.2
trnC-GCA-rpoB_p5	TCCAGTATCGCCAGACCTAG	CCCCACCTACACAAGCAAAT	55.0	50.0	58.0	58.1	1888-2043 bp	0.857143	78.8