Hotspot

trnY-GUA-trnD-GUC

LSC · 16489-16811 · 323 bp

Back to Panicum

Pi 0.0398
MarkerSeek score 80.90
Primer available yes
Label trnY-GUA-trnD-GUC

Feature Metadata

label_name
trnY-GUA-trnD-GUC
feature_type
igs
region
LSC
length_bp
323
variable_sites
37
pi
0.0398
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnD-GUC_p1 AATTCTTCCTGGGTCGATGC CGGTGCTCTGACCAATTGAA 50.0 50.0 58.0 58.5 447-479 bp 1.0 86.0
trnY-GUA-trnD-GUC_p2 GGGGACGGACTGTAAATTCG CGGTGCTCTGACCAATTGAA 55.0 50.0 58.4 58.5 414-446 bp 1.0 84.2
trnY-GUA-trnD-GUC_p3 GGGACGGACTGTAAATTCGT CGGTGCTCTGACCAATTGAA 50.0 50.0 57.6 58.5 413-445 bp 1.0 84.1
trnY-GUA-trnD-GUC_p4 AATTCTTCCTGGGTCGATGC GGTGCTCTGACCAATTGAAC 50.0 50.0 58.0 57.0 446-478 bp 1.0 81.9
trnY-GUA-trnD-GUC_p5 TGGGGACGGACTGTAAATTC CGGTGCTCTGACCAATTGAA 50.0 50.0 57.2 58.5 415-447 bp 1.0 80.9