Hotspot

trnfM-CAU-trnG-UCC

LSC · 12867-12960 · 94 bp

Back to Panicum

Pi 0.035
MarkerSeek score 73.30
Primer available yes
Label trnfM-CAU-trnG-UCC

Feature Metadata

label_name
trnfM-CAU-trnG-UCC
feature_type
igs
region
LSC
length_bp
94
variable_sites
10
pi
0.035
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-trnG-UCC_p1 CCTCAAGGTTATGAGCCTCG AGCCTTCCAAGCTAACGATG 55.0 50.0 57.8 58.0 184-185 bp 1.0 83.1
trnfM-CAU-trnG-UCC_p2 CCTCGTGAGCTACCAAACTG AGCCTTCCAAGCTAACGATG 55.0 50.0 58.3 58.0 169-170 bp 1.0 83.1
trnfM-CAU-trnG-UCC_p3 CCTCAAGGTTATGAGCCTCG CCTAGCCTTCCAAGCTAACG 55.0 55.0 57.8 58.1 187-188 bp 1.0 82.8
trnfM-CAU-trnG-UCC_p4 CCTCGTGAGCTACCAAACTG CCTAGCCTTCCAAGCTAACG 55.0 55.0 58.3 58.1 172-173 bp 1.0 82.8
trnfM-CAU-trnG-UCC_p5 CCTCAAGGTTATGAGCCTCG CCAACGTCGACTATAACCCC 55.0 55.0 57.8 57.8 205-206 bp 1.0 81.1