Hotspot

ndhE

SSC · 122870-123172 · 303 bp

Back to Pandanus

Pi 0.0066

MarkerSeek score 41.80

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 303
variable_sites: 3
pi: 0.0066
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	GGGTCACTTCACTGGTTTGT	AACACCAGACGAATCGATGG	50.0	50.0	57.9	58.0	950-1033 bp	1.0	84.9
ndhE_p2	GGGTCACTTCACTGGTTTGT	CACCAGACGAATCGATGGTT	50.0	50.0	57.9	58.0	948-1031 bp	1.0	84.9
ndhE_p3	GGGTCACTTCACTGGTTTGT	CTTAGCTTTACACCCGTCCC	50.0	55.0	57.9	58.0	1145-1228 bp	1.0	84.0
ndhE_p4	GGGTCACTTCACTGGTTTGT	AGCCTACACAGTCCTCAGTT	50.0	50.0	57.9	58.0	1185-1268 bp	1.0	83.9
ndhE_p5	GGGTCACTTCACTGGTTTGT	TCAAGAAATCCGTTGGGCAA	50.0	45.0	57.9	58.0	1224-1307 bp	1.0	83.8