Hotspot

ndhE-psaC

SSC · 123173-123541 · 369 bp

Back to Pandanus

Pi 0.0247

MarkerSeek score 60.90

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: SSC
length_bp: 369
variable_sites: 10
pi: 0.0247
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	TCAACTCGTATCAATCAATCGA	CTTAGCTTTACACCCGTCCC	36.4	55.0	55.4	58.0	477-525 bp	1.0	48.7
ndhE-psaC_p2	TCAACTCGTATCAATCAATCGA	TTACACCCGTCCCAAGGTAT	36.4	50.0	55.4	58.0	470-518 bp	1.0	48.5
ndhE-psaC_p3	TCAACTCGTATCAATCAATCGA	TGCTTAGCTTTACACCCGTC	36.4	50.0	55.4	57.9	479-527 bp	1.0	48.2
ndhE-psaC_p4	TCAACTCGTATCAATCAATCGA	TTTACACCCGTCCCAAGGTA	36.4	50.0	55.4	58.3	471-519 bp	1.0	46.7
ndhE-psaC_p5	TCAACTCGTATCAATCAATCGAA	CTTAGCTTTACACCCGTCCC	34.8	55.0	56.1	58.0	477-525 bp	1.0	46.5