Hotspot

trnR-ACG-trnN-GUU

IRb · 110526-111116 · 591 bp

Back to Pandanus

Pi 0.00457

MarkerSeek score 62.70

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 591
variable_sites: 4
pi: 0.00457
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	728-732 bp	1.0	79.6
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	CGGTTGGCTGTTAACTGACT	55.0	50.0	57.8	58.1	698-702 bp	1.0	78.7
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	692-696 bp	1.0	78.5
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	679-683 bp	1.0	78.4
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	CTGGTCGTAGGTTCGAATCC	55.0	55.0	57.8	57.8	680-684 bp	1.0	78.0