Hotspot

trnW-CCA-trnP-UGG

LSC · 68068-68248 · 181 bp

Back to Pandanus

Pi 0.0147
MarkerSeek score 62.80
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
181
variable_sites
4
pi
0.0147
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GCGTTCTACCGAACTGAACT GGGTACAAAATGTCACGGGT 50.0 50.0 57.9 58.1 233-250 bp 1.0 84.0
trnW-CCA-trnP-UGG_p2 GCGTTCTACCGAACTGAACT TGGGTACAAAATGTCACGGG 50.0 50.0 57.9 58.1 234-251 bp 1.0 84.0
trnW-CCA-trnP-UGG_p3 GCGTTCTACCGAACTGAACT GTCACGGGTTCAAATCCTGT 50.0 50.0 57.9 58.1 222-239 bp 1.0 84.0
trnW-CCA-trnP-UGG_p4 GCGTTCTACCGAACTGAACT CACGGGTTCAAATCCTGTCA 50.0 50.0 57.9 58.1 220-237 bp 1.0 84.0
trnW-CCA-trnP-UGG_p5 GCGTTCTACCGAACTGAACT TGTCACGGGTTCAAATCCTG 50.0 50.0 57.9 58.1 223-240 bp 1.0 84.0