Hotspot

petA-psbJ

LSC · 64281-65382 · 1102 bp

Back to Pandanus

Pi 0.0176

MarkerSeek score 67.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1102
variable_sites: 29
pi: 0.0176
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TTACGCGTCCAAGGTCTTTT	CGGGTTGGGTTCATCTCTTT	45.0	50.0	57.8	57.8	1229-1268 bp	1.0	80.1
petA-psbJ_p2	CATTACGCGTCCAAGGTCTT	CGGGTTGGGTTCATCTCTTT	50.0	50.0	58.3	57.8	1231-1270 bp	1.0	79.8
petA-psbJ_p3	TTACGCGTCCAAGGTCTTTT	GGTTCATATTCCGGGTTGGG	45.0	55.0	57.8	58.3	1240-1279 bp	1.0	79.2
petA-psbJ_p4	CATTACGCGTCCAAGGTCTT	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.3	58.3	1242-1281 bp	1.0	78.9
petA-psbJ_p5	GCGTCCAAGGTCTTTTGTTC	CGGGTTGGGTTCATCTCTTT	50.0	50.0	57.6	57.8	1225-1264 bp	1.0	78.9