Hotspot

accD-psaI

LSC · 59864-60600 · 737 bp

Back to Pandanus

Pi 0.0612

MarkerSeek score 89.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 737
variable_sites: 65
pi: 0.0612
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 89.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCACGGTTTCTTTCCCTTGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	847-880 bp	1.0	87.9
accD-psaI_p2	TCCACGGTTTCTTTCCCTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	848-881 bp	1.0	87.9
accD-psaI_p3	CCACGGTTTCTTTCCCTTGA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	853-886 bp	1.0	87.2
accD-psaI_p4	TCCACGGTTTCTTTCCCTTG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	854-887 bp	1.0	87.2
accD-psaI_p5	TCAGCTCCACGGTTTCTTTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	853-886 bp	1.0	87.0