Hotspot

psbM-trnD-GUC

LSC · 29750-30789 · 1040 bp

Back to Pandanus

Pi 0.00582

MarkerSeek score 62.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1040
variable_sites: 9
pi: 0.00582
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TATGCCTCACCGGGATTGTA	45.0	50.0	55.8	58.2	1165-1200 bp	1.0	61.8
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	CCTCACCGGGATTGTAGTTC	45.0	55.0	55.8	57.7	1161-1196 bp	1.0	61.0
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	CATATGCCTCACCGGGATTG	45.0	55.0	55.8	58.5	1167-1202 bp	1.0	59.7
psbM-trnD-GUC_p4	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1144-1179 bp	1.0	59.7
psbM-trnD-GUC_p5	AGGAACTAGAATGAACAGTGCA	TATGCCTCACCGGGATTGTA	40.9	50.0	57.4	58.2	1172-1207 bp	1.0	58.7