Hotspot

psbK-psbI

LSC · 7341-7683 · 343 bp

Back to Pandanus

Pi 0.0117

MarkerSeek score 61.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 343
variable_sites: 6
pi: 0.0117
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCAGGATTACG	50.0	50.0	57.9	57.1	480-489 bp	1.0	73.2
psbK-psbI_p2	AATCGTAGACGTTATGCCCG	TCTTCACGTCCAGGATTACG	50.0	50.0	57.9	57.1	532-541 bp	1.0	72.5
psbK-psbI_p3	ATGCCCGTCATACCTGTACT	TCTTCACGTCCAGGATTACG	50.0	50.0	58.2	57.1	519-528 bp	1.0	71.9
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	477-486 bp	1.0	71.2
psbK-psbI_p5	TTGGCAAGCTGCTGTAAGTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	478-487 bp	1.0	71.2