Hotspot

rps16-trnQ-UUG

LSC · 5802-6749 · 948 bp

Back to Pandanus

Pi 0.0121
MarkerSeek score 52.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
948
variable_sites
17
pi
0.0121
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCCGATCATGTCCTTCAA GAAATTGAAATGGGGCGTGG 50.0 50.0 58.0 58.0 1097-1188 bp 1.0 81.5
rps16-trnQ-UUG_p2 GCCGATCATGTCCTTCAAGT GAAATTGAAATGGGGCGTGG 50.0 50.0 58.0 58.0 1095-1186 bp 1.0 81.5
rps16-trnQ-UUG_p3 AATCCACAGCCGATCATGTC GAAATTGAAATGGGGCGTGG 50.0 50.0 58.0 58.0 1103-1194 bp 1.0 81.4
rps16-trnQ-UUG_p4 GAATCCACAGCCGATCATGT GAAATTGAAATGGGGCGTGG 50.0 50.0 58.0 58.0 1104-1195 bp 1.0 81.4
rps16-trnQ-UUG_p5 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1039-1130 bp 1.0 81.1