Hotspot

ycf1

SSC · 128861-134311 · 5451 bp

Back to Pancratium

Pi 0.00624

MarkerSeek score 49.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5451
variable_sites: 34
pi: 0.00624
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTAGTAATTTGATTGGTATGATCC	ACGCATATCGACACCAAGAT	29.2	45.0	52.6	57.1	262 bp	1.0	41.5
ycf1_p2	TTAGTAATTTGATTGGTATGATCC	ACGCATATCGACACCAAGATT	29.2	42.9	52.6	57.8	262 bp	1.0	41.5
ycf1_p3	ATTAGTAATTTGATTGGTATGATCC	ACGCATATCGACACCAAGAT	28.0	45.0	53.2	57.1	263 bp	1.0	41.5
ycf1_p4	TTAGTAATTTGATTGGTATGATCC	TACGCATATCGACACCAAGA	29.2	45.0	52.6	56.5	263 bp	1.0	41.5
ycf1_p5	AATTAGTAATTTGATTGGTATGATCC	ACGCATATCGACACCAAGAT	26.9	45.0	54.0	57.1	264 bp	1.0	41.5