Hotspot

psbC

LSC · 35370-36791 · 1422 bp

Back to Pancratium

Pi 0.00211

MarkerSeek score 48.30

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 3
pi: 0.00211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GCAGCGGAAGATCCTGAATT	TAGCCGAGCCAGAACAGAAA	50.0	50.0	58.3	59.0	1561 bp	1.0	69.3
psbC_p2	AGGTATTCGTGCTTGGATGG	TAGCCGAGCCAGAACAGAAA	50.0	50.0	57.7	59.0	1505 bp	1.0	69.3
psbC_p3	GCAGCGGAAGATCCTGAATT	GCCGAGCCAGAACAGAAAAA	50.0	50.0	58.3	59.0	1559 bp	1.0	69.2
psbC_p4	AGGTATTCGTGCTTGGATGG	GCCGAGCCAGAACAGAAAAA	50.0	50.0	57.7	59.0	1503 bp	1.0	69.1
psbC_p5	GCAGCGGAAGATCCTGAATT	TAGCCGAGCCAGAACAGAA	50.0	52.6	58.3	58.3	1561 bp	1.0	66.9