Hotspot

ndhD

SSC · 120377-121879 · 1503 bp

Back to Pancratium

Pi 0.00532

MarkerSeek score 50.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 8
pi: 0.00532
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGACTTTGCTTTCTGTCTCA	TGGCCAAAACGTATCTTGTCT	40.0	42.9	54.5	57.9	1564 bp	1.0	43.6
ndhD_p2	AAGACTTTGCTTTCTGTCTCA	TGGCCAAAACGTATCTTGTCT	38.1	42.9	55.4	57.9	1565 bp	1.0	42.3
ndhD_p3	AGACTTTGCTTTCTGTCTCAT	TGGCCAAAACGTATCTTGTCT	38.1	42.9	55.2	57.9	1564 bp	1.0	40.5
ndhD_p4	AGACTTTGCTTTCTGTCTCA	TGGCCAAAACGTATCTTGTC	40.0	45.0	54.5	56.4	1564 bp	1.0	40.4
ndhD_p5	AGACTTTGCTTTCTGTCTCATT	TGGCCAAAACGTATCTTGTCT	36.4	42.9	55.9	57.9	1564 bp	1.0	40.4