Hotspot

psaC-ndhE

SSC · 122245-122674 · 430 bp

Back to Pancratium

Pi 0.0165
MarkerSeek score 51.20
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
430
variable_sites
7
pi
0.0165
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 TTTCTAGTAACCGGCCAAGC GGGTCACTTCACTGGTTTGT 50.0 50.0 57.9 57.9 1842-1847 bp 1.0 79.7
psaC-ndhE_p2 ACATCCGTCCCAAGGTATCA GGGTCACTTCACTGGTTTGT 50.0 50.0 58.1 57.9 1250-1255 bp 1.0 79.7
psaC-ndhE_p3 TTTCTAGTAACCGGCCAAGC GGAAGCAGCTATTGGGCTAG 50.0 55.0 57.9 58.1 1210-1215 bp 1.0 79.6
psaC-ndhE_p4 TGAAGAACCCGCGAATATCG GGGTCACTTCACTGGTTTGT 50.0 50.0 58.2 57.9 1573-1578 bp 1.0 79.3
psaC-ndhE_p5 TTTCTAGTAACCGGCCAAGC GTTGGAGATGGGGTCACTTC 50.0 55.0 57.9 57.9 1852-1857 bp 1.0 79.2