Hotspot

ccsA-ndhD

SSC · 120126-120376 · 251 bp

Back to Pancratium

Pi 0.0518

MarkerSeek score 81.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 251
variable_sites: 13
pi: 0.0518
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGGTACGAATTCGGCACTTG	TGACAAAGTGCAGGCTATCC	50.0	50.0	58.3	57.9	407-412 bp	1.0	80.4
ccsA-ndhD_p2	CAAGGTACGAATTCGGCACT	TGACAAAGTGCAGGCTATCC	50.0	50.0	58.3	57.9	409-414 bp	1.0	80.4
ccsA-ndhD_p3	AAGGTACGAATTCGGCACTT	TGACAAAGTGCAGGCTATCC	45.0	50.0	57.5	57.9	408-413 bp	1.0	78.9
ccsA-ndhD_p4	GGTACGAATTCGGCACTTGT	TGACAAAGTGCAGGCTATCC	50.0	50.0	58.6	57.9	406-411 bp	1.0	78.1
ccsA-ndhD_p5	AGGTACGAATTCGGCACTTG	GACAAAGTGCAGGCTATCCT	50.0	50.0	58.3	57.6	406-411 bp	1.0	78.0