Hotspot

rpl32-trnL-UAG

SSC · 118232-119007 · 776 bp

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Pi 0.0142
MarkerSeek score 55.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
776
variable_sites
11
pi
0.0142
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTGCGACAAACAAGTAAGA TCCTAAGAGCAGCGTGTCTA 40.0 50.0 55.6 57.9 849 bp 1.0 60.5
rpl32-trnL-UAG_p2 TGTGCGACAAACAAGTAAGA AGCAGCGTGTCTACCAATTT 40.0 45.0 55.6 57.8 842 bp 1.0 60.0
rpl32-trnL-UAG_p3 TGTGCGACAAACAAGTAAGA CCTAAGAGCAGCGTGTCTAC 40.0 55.0 55.6 57.8 848 bp 1.0 60.0
rpl32-trnL-UAG_p4 TGTGCGACAAACAAGTAAGA CTAAGAGCAGCGTGTCTACC 40.0 55.0 55.6 57.8 847 bp 1.0 60.0
rpl32-trnL-UAG_p5 TGTGCGACAAACAAGTAAGA GAGCAGCGTGTCTACCAATT 40.0 50.0 55.6 58.3 843 bp 1.0 59.3