Pi
0.0116
MarkerSeek score
70.20
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1000-1047 bp | 1.0 | 83.7 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 1008-1055 bp | 1.0 | 83.1 |
| trnT-UGU-trnL-UAA_p3 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1032-1079 bp | 1.0 | 82.7 |
| trnT-UGU-trnL-UAA_p4 | GCGATGCTCTAACCTCTGAG | GGAAGTTCCCACTTAGCAGG | 55.0 | 55.0 | 57.9 | 57.9 | 1061-1108 bp | 1.0 | 82.5 |
| trnT-UGU-trnL-UAA_p5 | GGAATCGAACCGATGACCAT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 1040-1087 bp | 1.0 | 82.1 |