Hotspot

psbM-trnD-GUC

LSC · 30850-31868 · 1019 bp

Back to Pancratium

Pi 0.00688

MarkerSeek score 62.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1019
variable_sites: 7
pi: 0.00688
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	GTATGCTATACACCTCGCCG	45.0	55.0	55.8	58.0	1158-1159 bp	1.0	62.5
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	45.0	50.0	55.8	57.9	1131-1132 bp	1.0	62.0
psbM-trnD-GUC_p3	AGGAACTAGAATGAACAGTGCA	GTATGCTATACACCTCGCCG	40.9	55.0	57.4	58.0	1165-1166 bp	1.0	59.4
psbM-trnD-GUC_p4	AGGAACTAGAATGAACAGTGCA	AGTTCAATCGGTCAGAGCAC	40.9	50.0	57.4	57.9	1138-1139 bp	1.0	58.9
psbM-trnD-GUC_p5	GGAACTAGAATGAACAGTGCAG	GTATGCTATACACCTCGCCG	45.5	55.0	57.1	58.0	1164-1165 bp	1.0	56.9