Hotspot

trnC-GCA-petN

LSC · 28636-29693 · 1058 bp

Back to Pancratium

Pi 0.0161

MarkerSeek score 64.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1058
variable_sites: 17
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTA	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	57.5	58.0	1139-1144 bp	1.0	77.0
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTA	AGTCCACTCCTTCCCCATAC	50.0	55.0	57.5	58.1	1191-1196 bp	1.0	76.1
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTA	GTCCACTCCTTCCCCATACT	50.0	55.0	57.5	58.1	1190-1195 bp	1.0	76.1
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTA	GAGTCCACTCCTTCCCCATA	50.0	55.0	57.5	57.8	1192-1197 bp	1.0	75.6
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTA	CAGCCCAAGCAAGACTTACT	50.0	50.0	57.5	57.8	1133-1138 bp	1.0	75.5