Hotspot

psbC

LSC · 35884-37305 · 1422 bp

Back to Panax

Pi 0.0032
MarkerSeek score 55.50
Primer available yes
Label psbC

Feature Metadata

label_name
psbC
feature_type
gene
region
LSC
length_bp
1422
variable_sites
18
pi
0.0032
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC_p1 TTGGATGGCAGCTCAAGATC TGACCTGATTCCAATATGATGG 50.0 40.9 58.0 55.6 1510 bp 0.866667 40.0
psbC_p2 CTTGGATGGCAGCTCAAGAT TGACCTGATTCCAATATGATGG 50.0 40.9 57.9 55.6 1511 bp 0.866667 40.0
psbC_p3 AGGTATTCGTGCTTGGATGG TGACCTGATTCCAATATGATGG 50.0 40.9 57.7 55.6 1522 bp 0.866667 37.8
psbC_p4 CGGCGGAAGATCCTGAATTT TGACCTGATTCCAATATGATGG 50.0 40.9 58.3 55.6 1577 bp 0.866667 37.7
psbC_p5 TATTCGTGCTTGGATGGCAG TGACCTGATTCCAATATGATGG 50.0 40.9 58.3 55.6 1519 bp 0.866667 37.7