Hotspot

petB

LSC · 76876-78306 · 1431 bp

Back to Panax

Pi 0.00747
MarkerSeek score 44.50
Primer available yes
Label petB

Feature Metadata

label_name
petB
feature_type
gene
region
LSC
length_bp
1431
variable_sites
48
pi
0.00747
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB_p1 GACCGCGGAATTTCTTTGTT ACTATTGTTCTTCCTCCAAGT 45.0 38.1 57.3 54.2 1522-1532 bp 1.0 42.8
petB_p2 ATTTTGGTAGTTCGACCGCG ACTATTGTTCTTCCTCCAAGT 50.0 38.1 58.9 54.2 1535-1545 bp 1.0 42.8
petB_p3 TTCGACCGCGGAATTTCTTT ACTATTGTTCTTCCTCCAAGT 45.0 38.1 58.5 54.2 1525-1535 bp 0.933333 40.1
petB_p4 GTAGTTCGACCGCGGAATTT ACTATTGTTCTTCCTCCAAGT 50.0 38.1 58.6 54.2 1529-1535 bp 0.933333 39.9
petB_p5 TAGTTCGACCGCGGAATTTC ACTATTGTTCTTCCTCCAAGT 50.0 38.1 58.4 54.2 1528-1534 bp 0.866667 37.2