Hotspot

ndhF-rpl32

SSC · 114409-115626 · 1218 bp

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Pi 0.0119
MarkerSeek score 57.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1218
variable_sites
65
pi
0.0119
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.96
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGTCGGAAAAAGAAG TCCAAATATTTTTACGAATACGCT 50.0 29.2 58.0 55.3 1325-1377 bp 1.0 48.0
ndhF-rpl32_p2 TGCCGTCGGAAAAAGAAGAA TCCAAATATTTTTACGAATACGCT 45.0 29.2 58.0 55.3 1323-1375 bp 1.0 48.0
ndhF-rpl32_p3 TTGCCGTCGGAAAAAGAAGA TCCAAATATTTTTACGAATACGCT 45.0 29.2 58.0 55.3 1324-1376 bp 1.0 48.0
ndhF-rpl32_p4 TTTGTTGCCGTCGGAAAAAG TCCAAATATTTTTACGAATACGCT 45.0 29.2 57.8 55.3 1328-1380 bp 1.0 48.0
ndhF-rpl32_p5 GTTGCCGTCGGAAAAAGAAG TCCAAATATTTTTACGAATACGCTT 50.0 28.0 58.0 56.0 1325-1377 bp 1.0 48.0