Hotspot

petN-psbM

LSC · 30083-30944 · 862 bp

Back to Panax

Pi 0.00831
MarkerSeek score 56.40
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
862
variable_sites
38
pi
0.00831
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTTGCTTGGGCTGCTTTAA TGCTACTGCACTGTTCATTCT 45.0 42.9 58.0 57.6 1020-1036 bp 1.0 73.0
petN-psbM_p2 AGTAAGTCTTGCTTGGGCTG TGCTACTGCACTGTTCATTCT 50.0 42.9 57.8 57.6 1026-1042 bp 1.0 71.5
petN-psbM_p3 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.6 57.6 966-982 bp 1.0 70.4
petN-psbM_p4 TCTTGCTTGGGCTGCTTTAA TGCTACTGCACTGTTCATTC 45.0 45.0 58.0 56.1 1020-1036 bp 1.0 68.8
petN-psbM_p5 TCTTGCTTGGGCTGCTTTAA AGTTCCTACTGCTTTTCTGCT 45.0 42.9 58.0 57.2 999-1015 bp 0.933333 67.3