Hotspot

tRNA-Lys (UUU)-rps16

LSC · 4350-5243 · 894 bp

Back to Panax

Pi 0.00984
MarkerSeek score 64.50
Primer available yes
Label tRNA-Lys (UUU)-rps16

Feature Metadata

label_name
tRNA-Lys (UUU)-rps16
feature_type
igs
region
LSC
length_bp
894
variable_sites
41
pi
0.00984
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Lys (UUU)-rps16_p1 CGAATCCTTCGCTTCATCCA TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.1 58.3 1006-1086 bp 1.0 83.9
tRNA-Lys (UUU)-rps16_p2 CCGATGGTATGGACGAATCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 1019-1099 bp 1.0 83.6
tRNA-Lys (UUU)-rps16_p3 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 958-1038 bp 1.0 83.3
tRNA-Lys (UUU)-rps16_p4 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 957-1037 bp 1.0 83.3
tRNA-Lys (UUU)-rps16_p5 TCAGTCGTGGTCTTCCAAAC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 1043-1123 bp 0.933333 79.4