Hotspot

ycf92

LSC · 138538-139332 · 795 bp

Back to Palmaria

Pi 0.0994

MarkerSeek score 79.00

Primer available yes

Label ycf92

Feature Metadata

label_name: ycf92
feature_type: gene
region: LSC
length_bp: 795
variable_sites: 79
pi: 0.0994
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf92_p1	GCCTTTGGCTCTTCCTTCTT	TGCGTATACCAATTTCGCCA	50.0	45.0	58.1	58.0	1174-1175 bp	1.0	86.8
ycf92_p2	GCCTTTGGCTCTTCCTTCTT	TGTCTCCCCAATGTACCTGT	50.0	50.0	58.1	58.0	1359-1360 bp	1.0	86.2
ycf92_p3	TATCGAAGACGGCGTCTACT	TGCGTATACCAATTTCGCCA	50.0	45.0	58.1	58.0	1423 bp	0.5	59.4
ycf92_p4	TATCGAAGACGGCGTCTACT	TGTCTCCCCAATGTACCTGT	50.0	50.0	58.1	58.0	1608 bp	0.5	59.3
ycf92_p5	TATCGAAGACGGCGTCTACT	TCTAACGCTCTCCTGACGAT	50.0	50.0	58.1	58.0	1629 bp	0.5	59.2