Hotspot

ycf23

LSC · 165076-165924 · 849 bp

Back to Palmaria

Pi 0.0754

MarkerSeek score 76.90

Primer available yes

Label ycf23

Feature Metadata

label_name: ycf23
feature_type: gene
region: LSC
length_bp: 849
variable_sites: 64
pi: 0.0754
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf23_p1	AGAACCTACGGTAAGCCCTT	CCACCAGCAGAGGTTGATAC	50.0	55.0	58.1	58.0	1645 bp	1.0	85.3
ycf23_p2	AGAACCTACGGTAAGCCCTT	CCAGCAGAGGTTGATACACC	50.0	55.0	58.1	58.0	1642 bp	1.0	85.3
ycf23_p3	ATCCTGTCAGCTTGCTTGAG	CCACCAGCAGAGGTTGATAC	50.0	55.0	57.9	58.0	1677 bp	0.5	58.9
ycf23_p4	ATCCTGTCAGCTTGCTTGAG	CCAGCAGAGGTTGATACACC	50.0	55.0	57.9	58.0	1674 bp	0.5	58.9
ycf23_p5	CCTGTCAGCTTGCTTGAGAA	CCAGCAGAGGTTGATACACC	50.0	55.0	58.1	58.0	1672 bp	0.5	58.9