Hotspot

orf768

LSC · 141706-144012 · 2307 bp

Back to Palmaria

Pi 0.0832

MarkerSeek score 75.10

Primer available yes

Label orf768

Feature Metadata

label_name: orf768
feature_type: gene
region: LSC
length_bp: 2307
variable_sites: 192
pi: 0.0832
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf768_p1	CTCCTTGCTGATGCTCAACT	AGGAAATTGCTCGAGACGTC	50.0	50.0	57.9	58.0	2690 bp	1.0	86.2
orf768_p2	TGAGTCATCCATTGGCCATG	AGGAAATTGCTCGAGACGTC	50.0	50.0	57.9	58.0	2403 bp	0.5	59.5
orf768_p3	TGAGTCATCCATTGGCCATG	ACTTCTGCATGTGGGTCAAG	50.0	50.0	57.9	58.1	2571 bp	0.5	58.7
orf768_p4	TGAGTCATCCATTGGCCATG	CTGCATGTGGGTCAAGTTCT	50.0	50.0	57.9	58.1	2567 bp	0.5	58.7
orf768_p5	TGAGTCATCCATTGGCCATG	CTTCTGCATGTGGGTCAAGT	50.0	50.0	57.9	58.1	2570 bp	0.5	58.7