Hotspot

chlB

LSC · 96360-99834 · 3475 bp

Back to Palmaria

Pi 0.0636

MarkerSeek score 70.60

Primer available yes

Label chlB

Feature Metadata

label_name: chlB
feature_type: gene
region: LSC
length_bp: 3475
variable_sites: 221
pi: 0.0636
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB_p1	CTCAGGGATGCTAAAGCGTT	GTCCTATCGCTTGCTTCCAA	50.0	50.0	58.0	58.0	1132 bp	0.5	59.6
chlB_p2	CTCAGGGATGCTAAAGCGTT	ACTTTTTCTTGCGAACCCCT	50.0	45.0	58.0	57.9	937 bp	0.5	59.2
chlB_p3	CTCAGGGATGCTAAAGCGTT	CGGTCTCTTTCCAGCATTGA	50.0	50.0	58.0	57.9	868 bp	0.5	59.0
chlB_p4	CTCAGGGATGCTAAAGCGTT	GCTGCCCTGTCTACAAAGTT	50.0	50.0	58.0	58.1	1054 bp	0.5	58.9
chlB_p5	ATCTTAGCCTCAGGGATGCT	GTCCTATCGCTTGCTTCCAA	50.0	50.0	57.9	58.0	1140 bp	0.5	58.9