Hotspot

atpB

LSC · 115009-116433 · 1425 bp

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Pi 0.0491

MarkerSeek score 75.50

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1425
variable_sites: 70
pi: 0.0491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	GTCTGGTGCAATCACTCGTA	TGTCTTTTTACATTCATTGCTCA	50.0	30.4	57.6	55.1	1528-1530 bp	1.0	43.7
atpB_p2	GTCTGGTGCAATCACTCGTA	GTTGTCTTTTTACATTCATTGCTCA	50.0	32.0	57.6	57.1	1530-1532 bp	1.0	43.7
atpB_p3	GTCTGGTGCAATCACTCGTA	TGAGTTGTCTTTTTACATTCATTGC	50.0	32.0	57.6	57.1	1533-1535 bp	1.0	43.7
atpB_p4	TCTGTCTGGTGCAATCACTC	TGTCTTTTTACATTCATTGCTCA	50.0	30.4	57.5	55.1	1531-1533 bp	1.0	43.7
atpB_p5	GTCTGGTGCAATCACTCGTA	CTGAGTTGTCTTTTTACATTCATTGC	50.0	34.6	57.6	58.0	1534-1536 bp	1.0	43.6