Hotspot

orf88-apcF

LSC · 157815-158374 · 560 bp

Back to Palmaria

Pi 0.114

MarkerSeek score 68.20

Primer available yes

Label orf88-apcF

Feature Metadata

label_name: orf88-apcF
feature_type: igs
region: LSC
length_bp: 560
variable_sites: 34
pi: 0.114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.53
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf88-apcF_p1	CCACTGAGTTACATGCCCTC	AAGTGTCTCGCAATCCATCC	55.0	50.0	58.0	58.0	1258 bp	0.5	59.5
orf88-apcF_p2	CCACTGAGTTACATGCCCTC	AAGAATTCCCACCGGGTCTA	55.0	50.0	58.0	58.0	1129 bp	0.5	59.5
orf88-apcF_p3	CCACTGAGTTACATGCCCTC	AGAATTCCCACCGGGTCTAA	55.0	50.0	58.0	58.0	1128 bp	0.5	59.5
orf88-apcF_p4	CCACTGAGTTACATGCCCTC	TAAGAATTCCCACCGGGTCT	55.0	50.0	58.0	58.0	1130 bp	0.5	59.5
orf88-apcF_p5	CTAAGCCACGGGAAAACGTA	AAGTGTCTCGCAATCCATCC	50.0	50.0	57.9	58.0	1521 bp	0.5	59.1