Hotspot

trnS(tga)-psaD

LSC · 95269-95720 · 452 bp

Back to Palmaria

Pi 0.195
MarkerSeek score 76.60
Primer available yes
Label trnS(tga)-psaD

Feature Metadata

label_name
trnS(tga)-psaD
feature_type
igs
region
LSC
length_bp
452
variable_sites
88
pi
0.195
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS(tga)-psaD_p1 CCGCAAATTACACGTGCATT GATGGTCGTGAAGCAGCTAA 45.0 50.0 58.0 58.0 1283 bp 0.5 59.7
trnS(tga)-psaD_p2 CCCGGATGAAGATGCTGAAA GATGGTCGTGAAGCAGCTAA 50.0 50.0 58.0 58.0 732 bp 0.5 59.6
trnS(tga)-psaD_p3 ACCAACTCTACCACCTCTCC GATGGTCGTGAAGCAGCTAA 55.0 50.0 58.1 58.0 568 bp 0.5 59.4
trnS(tga)-psaD_p4 CCGCAAATTACACGTGCATT TACGCAATGTTCCGATGTGT 45.0 45.0 58.0 57.9 1881 bp 0.5 59.1
trnS(tga)-psaD_p5 ATAGCAACACAGTTCCGCAA GATGGTCGTGAAGCAGCTAA 45.0 50.0 58.1 58.0 1297 bp 0.5 59.0