Hotspot

rpoC1

LSC · 21429-24179 · 2751 bp

Back to Paeonia

Pi 0.00564

MarkerSeek score 41.80

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2751
variable_sites: 70
pi: 0.00564
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GCTCGAATTCATACCATATCA	TGCTCGTTCGAGAATTACGA	38.1	45.0	53.3	57.1	2924-2939 bp	1.0	42.3
rpoC1_p2	GCTCGAATTCATACCATATCA	TTGCTCGTTCGAGAATTACGA	38.1	42.9	53.3	57.8	2925-2940 bp	1.0	42.3
rpoC1_p3	GCTCGAATTCATACCATATCAT	TGCTCGTTCGAGAATTACGA	36.4	45.0	53.9	57.1	2924-2939 bp	1.0	42.3
rpoC1_p4	GCTCGAATTCATACCATATCA	TGCTCGTTCGAGAATTACGAT	38.1	42.9	53.3	57.6	2924-2939 bp	1.0	42.3
rpoC1_p5	GCTCGAATTCATACCATATCA	TACGATCTTTGGCTCTGGAG	38.1	50.0	53.3	56.7	2909-2924 bp	1.0	42.2