Hotspot

ndhF-trnL-UAG

SSC · 112291-113309 · 1019 bp

Back to Paeonia

Pi 0.0132

MarkerSeek score 65.80

Primer available yes

Label ndhF-trnL-UAG

Feature Metadata

label_name: ndhF-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1019
variable_sites: 62
pi: 0.0132
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnL-UAG_p1	TTTGTTGCCGTCGGAAAAAG	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.8	57.9	793-1169 bp	1.0	86.0
ndhF-trnL-UAG_p2	TTTGTTGCCGTCGGAAAAAG	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.8	57.8	786-1162 bp	1.0	85.5
ndhF-trnL-UAG_p3	TTTGTTGCCGTCGGAAAAAG	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	57.8	57.8	792-1168 bp	1.0	85.4
ndhF-trnL-UAG_p4	TTTGTTGCCGTCGGAAAAAG	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.8	57.8	791-1167 bp	1.0	85.4
ndhF-trnL-UAG_p5	TTTGTTGCCGTCGGAAAAAG	GAAGATAAGATCCCGTGCCG	45.0	55.0	57.8	58.2	849-1225 bp	1.0	85.1