Hotspot

psaI-ycf4

LSC · 60209-60633 · 425 bp

Back to Paeonia

Pi 0.0111

MarkerSeek score 58.80

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 425
variable_sites: 22
pi: 0.0111
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	TGCCTTTGGTGGGTCTAGTA	TTGTTTTTCGAGATCCCGCT	50.0	45.0	58.0	57.8	522-566 bp	1.0	85.6
psaI-ycf4_p2	TTTCCAGCAATTGCAATGGC	TTGTTTTTCGAGATCCCGCT	45.0	45.0	58.2	57.8	502-546 bp	1.0	84.5
psaI-ycf4_p3	CAGCAATTGCAATGGCTTCT	TTGTTTTTCGAGATCCCGCT	45.0	45.0	57.6	57.8	498-542 bp	1.0	83.0
psaI-ycf4_p4	TGCCTTTGGTGGGTCTAGTA	CTTGTTTTTCGAGATCCCGC	50.0	50.0	58.0	57.5	523-567 bp	1.0	82.8
psaI-ycf4_p5	TTTCCAGCAATTGCAATGGC	CTTGTTTTTCGAGATCCCGC	45.0	50.0	58.2	57.5	503-547 bp	1.0	81.7